L-Isoleucine is an essential amino acid (which means that body cannot produce it on its own). It is the fourth most abundant amino acid in human skeletal muscle. Natural sources of L-Isoleucine include nuts, almonds, seeds, meat, cashews, eggs, brown rice, whole wheat, fish, chicken, liver, lentils, peas and soy proteins. L-Isoleucine is used in powdered form as a supplement by performance athletes and bodybuilders because it is shown to significantly boost energy. Research also shows that L-Isoleucine supplementation can boost endurance and aid in muscle recovery and repair after workouts. Deficiency of L-Isoleucine can cause headaches, dizziness, fatigue and depression.

L-Leucine is not prepared in the body but can be obtained through food items like brown rice, whole wheat bread and nuts or dietary supplements. It provides power and endurance. It ensures the well being of the lean muscle tissue, maintains the body’s nitrogen balance and supplies energy especially during exercise. It also assists the healing process and preserves and builds muscles. It is ideal for sportspersons. L-Leucine is also known to increase the secretion of Human Growth Hormone resulting in healthy joints, the reduction of body fat and slowing down of the aging process. It is also useful to people with Diabetes and kidney and liver problems. In the case of a deficiency, the body will not be able to use any proteins efficiently.

L-Valine is the third branched chain amino acid and is required for muscle metabolism, repairs and regenerates tissue, and maintains the body's nitrogen level. It also is used as a muscle energy source and preserves the use of glucose.